Proteomics

One set of Synechococcus sp. PCC 7002 phosphoproteome data has been collected from published literatures.

I. Introduction: Phosphorylation is of great importance for biochemical activities in cells and lots of protein phosphorylation analyses have been carried out. This research article focuses on protein phosphorylation phenomenon in Synechococcus sp. PCC 7002 and reveals the underlying physiological functions.( Corresponding Reference )

II. Data Access: A table containing all the identified phosphorylated proteins and their detailed information, including phosphorylated peptides, biological functions and functional categories they belong to is provied. For each protein, a list of links to our Genome Browser, search engine of CyanOmics, NCBI (National Center for Biotechnology Information) and CyanoBase databases is provided; users can choose any of these to access the corresponding page. Click the link below to see this table.

A table of all the phosphorylated proteins and their detailed information


Details about experimental methods and conditions in this dataset.

Protein extracts applied to phosphoproteomic analysis were isolated from cell cultures grown in medium A supplemented with 1 mg mL-1 NaNO3 as nitrogen source. The growth temperature was 38°C with continuous illumination at 250 μmol photons m(−2) s(−1) light intensity and 1% (v/v) CO2 in air. Cells were harvested at the early/midexponential phase. The specially treated proteins were subjected to Mass Spectrometry analysis and the phosphorylated amino acids (mainly Serine, Threonine and Tyrosine) were identified.

Two ways below are also provided to access the phosphoproteome data:

I. Browsing the track named " All Phosphorylated Peptides " in PCC 7002 Omics Browser. In the " Entry Detail " part, detailed information such as search engine names, scores, phosphorylation sites, peptide sequences and their corresponding nucleic acid sequences can be obtained.

II. Detailed information about the phosphoproteome data can be displayed using the search box in navigation bar or Cyanobase functional categorization tree on the CyanOmics homepage.